SARS-CoV-2 (COVID19) irakaze hirya no hino ku isi, kandi inkingo izwi nk'uburyo bwubukungu kandi bunoze bwo kurwanya icyorezo cya virusi.Isuzuma ryinkingo gakondo ahanini rikoresha uburyo bwo kutabuza antibody uburyo bwo gusuzuma imikorere yinkingo hakoreshejwe ubushakashatsi bwo kutabogama;
Uburyo gakondo butwara igihe kandi buke mubikorwa, mubisanzwe bifata iminsi 2 kugeza kuri 4 kugirango urangize isuzuma, kandi kubera ko inyinshi murizo zikoresha virusi nzima, Igomba gukorwa murwego rwa biosafeti kurwego rwa 3 cyangwa hejuru ya laboratoire, nigihe- kumara no gukora cyane, kandi bizana ingorane nyinshi mugusuzuma kwaguka no guteranya.Kubwibyo, harakenewe byihutirwa ubundi buryo bworoshye kandi bwihuse bwuburyo bukwiranye no gusuzuma antibodiyide zirinda abantu benshi.
Aehealth COVID19 Kutabogama Antibody Quantitative Test Kit ikoreshwa mugutahura kwinshi kwa COVID19 itabuza antibodi muri serumu yumuntu, plasma cyangwa mumaraso yose.Irashobora gukoreshwa muburyo bwihuse, bwinshi kandi bworoshye cyane muri vitro.mubuvuzi bukoreshwa mugusuzuma ubufasha bwingaruka zurukingo rushya rwa coronavirus no gusuzuma isuzuma rya antibodiyite ku barwayi bakize nyuma yo kwandura.
Kutabogama antibodies bihagarika neza kwandura muguhagarika imikoranire hagati ya virusi ya COVID19 na selile zakira.Antibodiyite nyinshi zidafite aho zibogamiye zisubiza reseptor ihuza domeni (RBD) ya proteine ya spike, ihuza neza na selile ACE2.antibodies-kumurongo kuri ubu itanga antibodies ebyiri zidafite aho zibogamiye zishingiye kuri clone CR3022.Mugihe antibodiyite nyinshi za S-protein RBD zihatanira guhuza antigen hamwe na ACE2, epitope CR3022 ntabwo ihura na site ya ACE2 ihuza.
Ntabwo rero bibuza guhuza antibodiyite.Mugihe CR3022 yonyine yerekana ingaruka zidafite aho zibogamiye, byagaragaye ko ihuza nizindi S-proteine RBD ihuza antibodiyite kugirango ibuze COVID19.
Igikorwa cyoroshye
- Ntabwo ukeneye abanyamwuga guhugurwa;
- Icyitegererezo gike gisabwa, gikeneye 50 μL gusa;
- Bihujwe nubwoko bwinshi bwikigereranyo: Serumu / Plasma / Amaraso yose.
Ubukangurambaga bukabije
- Ibyiyumvo: 98,95%;
- Umwihariko: 100%.
Ingirakamaro
- Igihe cyo kwitabira: iminota 15, Igihe cyikizamini: 10s;
- Kurindwa, Ikoreshwa ryinshi;
- Yubatswe muri bateri, ibizamini birenga 200 nta kwinjiza ingufu.
Yizewe
- Byagenzuwe n’ibizamini 3600 by’ibizamini, ibizamini 1500 n’icyitegererezo cy’umuntu wanduye, ibizamini 900 ku nkingo, ibizamini 1200 n’abantu basanzwe.;
- Amakuru y’amavuriro aboneka mu rukingo n’urukingo rudakora, urukingo rwa aside nucleique, urukingo rwa poroteyine, n’abanduye virusi, abantu basanzwe.
- Mugabanye igipimo cyo kubuza agaciro ka 30%.
Ihame ryibanze rikoreshwa muri iki gikoresho ni amarushanwa ya immunochromatografiya. Umurongo wo gutahura (T-umurongo) kumurongo wibizamini washyizweho na angiotensin uhindura enzyme 2 naho umurongo ugenzura (C-umurongo) ushyizwemo ihene irwanya urukwavu antibody. padi ya conjugate isizwe hamwe na poroteyine ya COVID19 RBD ya flfluorescent na flfluorescent yanditseho urukwavu IgG antibody.Mugihe cyo gutahura, mugihe icyitegererezo kirimo antibody igomba gupimwa, conjugate yibintu byipimishije murugero hamwe na antigen ya flfluorescent ikora urwego rwumubiri kandi urwego rwumubiri ntirushobora guhuza na enzyme ya angiotensin ihindura 2 ya immibilisation kuri membrane ya nitrocellulose. .Antigen conjugate ya flfluorescent idahujwe na antibody igomba gupimwa izahuza na angiotensin ihindura enzyme 2 ihindagurika kuri membrane ya nitrocellulose kugirango ibe umurongo wo kumenya (T).
- Ikizamini cyo gusuzuma mbere yo gukingirwa;
- Gukurikirana ibisubizo nyuma yo gukingirwa;
- Isuzuma ry'ingaruka ku banduye bwa kabiri banduye;
- Isuzuma ryibyago kubantu basanzwe (harimo kwandura asimptomatic) bashobora kwandura;
- Kwipimisha ubushobozi bwa virusi.