COVID19-Omicron

Igikoresho gikoreshwa muri vitro yujuje ubuziranenge bwo kumenya genes ya ORF1ab / N / S ya coronavirus ya 2019-nCoV mu myanya y'ubuhumekero harimo oropharyngeal swabs, nasopharyngeal swab, sputum no gukora mutation yandika kuri HV69- 70 del icyarimwe.Nkuko byavuzwe muri base ya GISAID, ibice birenga 95% bya Omicron byahinduwe bikubiyemo gusiba muri HV69-70, bishobora gutera intego yo kunanirwa na S gene (SGTF) mubisubizo bya PCR.SGTF irashobora gukoreshwa nkibimenyetso bya porokisi kugirango yerekanwe kuri Omicron.

Primer set na FAM yanditseho probe byateguwe kugirango hamenyekane neza gene ya laboratoire ya ORF ya 2019-nCoV, VIC yanditseho probe ya N gene ya 2019-nCoV, ROX yanditseho probe ya S gene HV69-70 del mutation ya 2019-nCoV.Gene ya RNase P yakuwe hamwe hamwe nicyitegererezo cyikizamini itanga igenzura ryimbere kugirango yemeze uburyo bwo gukuramo nucleic hamwe nubusugire bwa reagent.Probe yibasira gene ya RNase P yanditswemo CY5.