Iki gikoresho gikoreshwa muri vitro qualitative nucleic acide ya virusi ya Monkeypox hamwe na clade yo muri Afrika yo hagati / uburengerazuba muri serumu yumuntu, lesion exudate sample hamwe na scab.Primer set hamwe na FAM yanditseho probe yagenewe kumenya neza virusi ya Monkeypox, ROX yanditseho probe kumurongo wo muri Afrika yo hagati, CY5 yanditseho probe kuburengerazuba bwa Afrika.Gene ya RNase P yakuwe hamwe hamwe nicyitegererezo cyikizamini itanga igenzura ryimbere kugirango yemeze uburyo bwo gukuramo nucleic hamwe nubusugire bwa reagent.Probe yibasira gene muntu RNase P yanditseho VIC.
| Ibigize | 48 Ibizamini / ibikoresho | 96 Ibizamini / ibikoresho |
| PCR reaction ya buffer | 672 μL × 1 umuyoboro | 672 μL × 2 umuyoboro |
| Enzyme ya PCR ivanze | 50 μL × 1 umuyoboro | 100 μL × 1 umuyoboro |
| Kugenzura neza | 100 μL × 1 umuyoboro | 200 μL × 1 umuyoboro |
| Kugenzura nabi | 100 μL × 1 umuyoboro | 200 μL × 1 umuyoboro |
1. Ibyiyumvo: kopi 200 / mL.
2. Umwihariko: Nta reaction yambukiranya Enterovirus (EV), virusi ya Measles (MV), virusi ya Rubella (RV), virusi ya Varicella-zoster (VZV), virusi ya Dengue (DenV), abantu Parvovirus B19 (HPVB19), virusi ya Epstein-barr (EBv), virusi ya herpes ya muntu 6 (HHV-6)
3. Icyitonderwa: CV ≤ 5%.
Sisitemu nyayo PCR: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P / 3005P, Rotor-GeneTM 6000 / Q, Bio-Rad CFX96 TouchTM / iQTM 5 ， Hongshi SLAN -96S / 96P ， AGS8830, AGS4800