Iki gikoresho gikoreshwa muri vitro qualitative nucleic aside gutahuraVirusi ya Monkeypox muri serumu yumuntu, lesion exudate ingero naingero.Gushiraho primer na FAM yanditseho probe yarateguwekugirango tumenye neza virusi ya Monkeypox, Gene ya muntu RNase P.gukuramo icyarimwe hamwe nicyitegererezo cyikizamini gitanga imberekugenzura kugirango yemeze uburyo bwo gukuramo nucleic na reagentubunyangamugayo.Probe yibasira gene muntu RNase P yanditseho VIC.
| Ibigize | 48 Ibizamini / ibikoresho | 96 Ibizamini / ibikoresho |
| PCR reaction ya buffer | 672 μL × 1 umuyoboro | 672 μL × 2 umuyoboro |
| Enzyme ya PCR ivanze | 50 μL × 1 umuyoboro | 100 μL × 1 umuyoboro |
| Kugenzura neza | 100 μL × 1 umuyoboro | 200 μL × 1 umuyoboro |
| Kugenzura nabi | 100 μL × 1 umuyoboro | 200 μL × 1 umuyoboro |
1. Ibyiyumvo: kopi 200 / mL.
2. Umwihariko: Nta reaction yambuka hamwe na Enterovirus (EV), Indwaravirusi (MV), virusi ya Rubella (RV), virusi ya Varicella-zoster (VZV),Virusi ya Dengue (DenV), UmuntuParvovirus B19 (HPVB19),Virusi ya Epstein-barr (EBv), virusi ya herpes ya muntu 6 (HHV-6)
3. Icyitonderwa: CV ≤ 5%.
Sisitemu nyayo PCR: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P / 3005P, Rotor-GeneTM 6000 / Q, Bio-Rad CFX96 TouchTM / iQTM 5 ， Hongshi SLAN-96S / 96P ， AGS8830, AGS4800