Iki gikoresho gikoreshwa muri vitro qualitative nucleic acide ya 2019-Novel Coronavirus (2019-nCoV), ibicurane A na Grippe B icyarimwe mubihumekero harimo na oropharyngeal swabs na sputum.Primer set hamwe na FAM byanditseho probe byateguwe kugirango hamenyekane neza genes zombi za ORFlab na N zo muri 2019-nCoV, VIC yanditseho probe ya genase ya muntu RNase P nkigenzura ryimbere, ROX yanditseho probe ya M gene ya grippe A, CY5 yanditseho probe ya NS gene ibicurane B.
| Ibigize | Ibizamini 50 / kit |
| RT-PCR reaction buffer | 915 μL × 1 umuyoboro |
| RT-PCR enzyme ivanze | 75 μL × 1 umuyoboro |
| Kugenzura neza | 100 μL × 1 umuyoboro |
| Kugenzura nabi | 100 μL × 1 umuyoboro |
Ibyiyumvo copies 200 kopi / mL.
Umwihariko ： Umwihariko ： Nta reaction yambukiranya hamwe na SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63, virusi ya Parainfluenza, Umuntu Adenovirus, Rhinovirus Yumuntu, Virusi Yubuhumekero (A), Syncytial Syncytial Virusi (B), Enterovirus A, Staphylococcus Aureus, Streptococcus Pneumonia, Klebsiella Umusonga.
Icyitonderwa ： CV ≤ 5%.
Sisitemu nyayo-PCR:Ubuzima bwiza Diagenex AL,Molarray MA-6000, ABI 7500, ViiATM7, QuantStudio 7 flex, Roche Lightcycler 480, Agilent Mx3000P / 3005P, Rotor-GeneTM6000 / Q, Bio-Rad CFX96 GukorahoTM/ iQTM5 ， SLAN-96S / 96P