Spike glycoprotein reseptor-binding domaine (RBD) ya SARS-CoV-2 ihuza agace ka virusi ihuza na angiotensin ihindura enzyme 2 (ACE2) yakira hejuru yingirabuzimafatizo zabantu.Kubwibyo, imikoranire ya Spike-ACE2 nikintu gikomeye kigena kwandura virusi. Itsinda rishya rya phylogeneque rya SARS-CoV-2 (umurongo B.1.1.7), rigaragaza gusimbuza aside amine muri Spike RBD (N501Y mutation).
Nk’uko ubushakashatsi bushya bwakozwe n’ishami ry’ubuzima ry’Ubwongereza bubitangaza, bushingiye ku mibare ikurikirana ya genome yose uko yakabaye, epidemiologiya ndetse no kwerekana imiterere, ubwandu bw’imiterere ya SARS-CoV-2 buri hejuru ya 40-70% ugereranije n’abandi bahanga mu byahinduwe byavumbuwe. .Igisubizo cyihuse no gutahura nikimwe mubikorwa byingenzi byo gukumira no kugenzura.
Igikoresho gikoreshwa muri vitro yujuje ubuziranenge bwo kumenya genes ya ORF1ab / N / S ya coronavirus ya 2019-nCoV mu myanya y'ubuhumekero, no gukora mutation yanditse kuri N501Y icyarimwe.
Igikoresho gikoresha uburyo bwa ARMS, gifata S gene yubuvanganzo bwa coronavirus (2019-nCoV) nkakarere kagenewe kwandika mutation, igashushanya primers na probe kuri N501Y.
| Ibicuruzwa | Ibisobanuro | Ububiko temp | Ibisobanuro |
| Igihe nyacyo PCR Kit kubitaboCoronavirus 2019-nCoV naIgitabo Coronavirus 2019-nCoVGuhindura Gene (N501Y) | 48T | -20 ℃ | Isesengura: Ubwoko-bwa N501Y,Ubwoko bwa Mutant bwa N501Y, ORF1ab,N gene no kugenzura imbere |
Guhuza Inyandikorugero
- Oropharyngeal Swabs
- Nasopharyngeal Swab
- Amazi ya Bronchoalveolar
- Amacandwe
Ibigize ibikoresho
- RT-PCR reaction buffer
- RT-PCR enzyme ivanze
- Ubwoko bwiza bwo kugenzura
- Guhindura ibintu byiza
- Kugenzura nabi
N501Y Ubwoko bwa Mutation
