| 64T | 96T | ||
| Ibigize | Umubare | Ibigize | Umubare |
| Isahani | 4 | Lysis buffer B. | 2 |
| Lysis buffer B. | 1 | Isahani ya Lysis | 1 |
| Amaboko ya plastiki | 8 | Karaba isahani 1 | 1 |
| Igitabo cya protocole | 1 | Karaba isahani 2 | 1 |
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| Isahani | 1 |
|
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| Amaboko ya plastiki | 1 |
|
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| Igitabo cya protocole | 1 |
96-iriba Ruzengurutsa Isahani
64T Ibigize isahani ijyanye nibi bikurikira:
| Urubuga | 10r7 | 2or8 | 3or9 | 4or10 | 5orll | 6orl2 |
| Kit Ibigize | Lysis Buffer 600μL | Karaba Buffer1 500μL | Karaba Buffer2 500μL | Wibeshya | Magnetic Amasaro 310μL | Elute Buffer l00μL |
Fcyangwa 64T kit:
Witonze ukureho firime ifunga ubushyuhe ku isahani ya reagent, hanyuma wongereho 200μL yicyitegererezo na 20μL ya lysis buffer B muri 1/7 cyinkingi ya reagent.
Kuri 96T kit:
Witonze ukureho firime ifunga ubushyuhe kuri plaque ya reagent, hanyuma wongere 200μL yicyitegererezo na 20μL ya lysis buffer B muri plaque ya lysis.
Shyiramo isahani ya reagent hamwe na plastike ya plastike mumwanya wabigenewe kugirango ukurikirane, hanyuma ukande kugirango ukore gahunda yo gukuramo "ADN / RNA" kumashanyarazi acide nucleic.
Porogaramu irangiye, fata amaboko ya plastike uyijugunye.
Kuramo isahani ya elution, hanyuma eluent ikururwa ikabikwa mumiyoboro mishya ya centrifuge kugirango igerageze kumanuka.Niba igeragezwa ryo hasi ridashobora gukorwa mugihe, icyitegererezo cya ADN gishobora kubikwa kuri -20 ℃ naho icyitegererezo cya RNA gishobora kubikwa kuri -80 ℃.